System Composition

The aseptic cell preparation isolation system consists of an aseptic isolator, honeycomb cell culture system, transfer system and information management system.Installed inside the aseptic isolator are a centrifuge, pipetting system, liquid preparation system, sampling microscopic system, as well as sterilization system, residual vapor removal system, laminar flow system, control and monitoring system. The equipment supports both automatic robotic manipulator operation and manual manipulation via operating gloves.The honeycomb culture system comprises mobile incubators, CO2 incubation systems and honeycomb racks.

Description of the cell preparation process

• Connect the honeycomb incubator to the isolator, then inflate and seal it to secure and enclose the unit. Initiate hydrogen peroxide sterilization to disinfect the external surface of the incubator and remove any residual contaminants.
• Open the inner door of the honeycomb docking system using gloves, then open the door of the honeycomb incubator, remove the culture cell bottles, and scan the code to obtain cell information.
• On one hand, close the inner door of the docking chamber and activate the sterilization system to sterilize the incubator chamber. On the other hand, in the operating compartment, open the centrifuge door, place the culture flasks into the centrifuge, close the centrifuge door, and initiate centrifugation.
• After centrifugation is completed, open the centrifuge door, remove the centrifuged culture flasks, and place them in the pipetting module position.
• Initiate the waste liquid collection procedure of the pipetting module to draw the centrifuged waste liquid into the waste container, and transfer a small amount of cells onto a glass slide for microscopic examination and cell counter reading.
• Initiate the nutrient addition program of the pipetting module by adding sterile nutrient solution to the culture flask and rinsing the flask to dislodge cells from the walls into the culture medium, then divide one flask of cultured cells into two flasks.
• Cover the culture bottle containing the nutrient solution, return it to the honeycomb incubator, close the incubator door and the inner door of the docking chamber, release the inflation seal strip on the outer docking door, then move the incubator to the designated position for cultivation.
• Observe and examine the shape and quantity of cells using a microscope within the isolator, and record the information of this batch of cells in the system.
• After thoroughly cleaning the pipette and sampling specimen, wait for the next hive to arrive.
• After a period of culture, when the cell population has reached the desired quantity, the waste liquid can be discharged using the pipetting module, and the cultured cells can then be directly transferred out of the isolator and stored in a freezer for preservation.